Recently there has been a push to develop biomass polymeric films from natural resources such as polysaccharides, DNA, and proteins. It is well known that natural materials such as cellulose and silk fibers have incredible mechanical properties, good biological compatibility, and biodegradability. There are many uses for polymeric films, including the 3D printing of green plastics and bio-medical devices. Our main focus is to develop polymeric bio-films from various polysaccharides, such as cellulose and lignin, xylan, proteins, and ionic liquids using cost efficient techniques. After the films are produced, we study the structure property relationships using microscopic, macroscopic, spectroscopic and thermal analyses.